Transcription and Translation Reagents

A variety of kits, systems, buffers, inhibitors, RNA cap analogs, reagents, and solutions for use in experiments involving transcription and translation. Products can be used in the synthesis of RNA probes.

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Invitrogen™ mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit

Generate RNA transcripts with higher protein yields

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Invitrogen™ mMESSAGE mMACHINE™ T3 Transcription Kit

Obtain 10–50 times yield of conventional in vitro transcription reactions

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Invitrogen™ MEGAscript™ SP6 Transcription Kit

Synthesize 10 to 50 times the amount of RNA produced by conventional transcription reactions

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BD Pharmingen™ Transcription-Factor Buffer Set

Optimized for fixing and permeabilizing cells prior to immunofluorescent staining and flow cytometric analysis of cells that express specific intracytoplasmic and intranuclear proteins

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Promega TNT™ Coupled Wheat Germ Extract Systems

The TNT Coupled Wheat Germ Extract Systems are one-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.

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Specifications

Includes	1mL TNT™ Wheat Germ Extract, 90μL TNT™ Reaction Buffer, 60μL TNT™ RNA Polymerase (SP6, T3 or T7, individually or 2 x 30μL when two Polymerases are
No. of Reactions	40
Content And Storage	<-65°C
For Use With (Application)	Translation of mammalian transcription factors

Promega Flexi™ Rabbit Reticulocyte Lysate System

Flexi Rabbit Reticulocyte Lysate System allows optimization of translation reactions for a range of parameters, including Mg(2+) and K(+) and DTT concentration.

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Promega Transcend™ Nonradioactive Translation Detection Systems

Non-Radioactive Translation Detection Systems for colorimetric or chemiluminescent detection of proteins synthesized in vitro. Biotinylated proteins are detected using Streptavidin-Alkaline Phosphatase or Streptavidin-HRP.

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BD Human and Mouse Pluripotent Stem Cell Analysis Kit

To analyze cells for expression of cell surface and intracellular pluripotency markers and differentiation markers

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Invitrogen™ mMESSAGE mMACHINE™ SP6 Transcription Kit

in vitro synthesis of large amounts of capped RNA

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Invitrogen™ MEGAshortscript™ T7 Transcription Kit

Maximize the yield of short RNA transcripts

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Invitrogen™ mMESSAGE mMACHINE™ T7 Transcription Kit

in vitro synthesis of large amounts of capped RNA

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Invitrogen™ Cap Analog (m⁷G(5')ppp(5')G), 100 A₂₅₄ Units

For 5' capped RNA molecules synthesis as part of in vitro transcription reactions

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Invitrogen™ MAXIscript™ SP6/T7 Transcription Kit

Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations

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Specifications

Shipping Condition	Dry Ice
Promoter	T7, SP6
Content And Storage	SP6 Enzyme Mix, T7 Enzyme Mix,10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format	Kit
Product Type	SP6/T7 In Vitro Transcription kit
Final Product Type	Synthetic mRNA,mRNA
Sample Type	mRNA,Synthetic mRNA
Yield	2-6 µg
Starting Material	PCR Products, Linearized DNA plasmid
For Use With (Application)	In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line	Ambion™, MAXIscript™

Invitrogen™ MAXIscript™ T7/T3 Transcription Kit

Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Promoter	T3, T7
Content And Storage	T7 Enzyme Mix, T3 Enzyme Mix,10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Format	Kit
Product Type	T7/T3 In Vitro Transcription kit
Final Product Type	Synthetic mRNA,mRNA
Sample Type	mRNA,Synthetic mRNA
Yield	2-6 µg
Starting Material	PCR Products, Linearized DNA plasmid
For Use With (Application)	In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Product Line	Ambion™, MAXIscript™

Invitrogen™ MEGAscript™ T7 Transcription Kit

Ultra-high yield technology for in vitro transcription

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Transcription and Translation Reagents

Transcription and Translation Reagents

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