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Thermo Scientific™ Pierce™ GST Agarose
Isolate or remove cross-reacting GST-specific serum or other antibody solutions with immobilized glutathione S-transferase agarose.
Supplier: Thermo Scientific™ 20211
Description
Perform isolation or removal of cross-reacting GST-specific antibodies from serum or other antibody solutions with Thermo Scientific™ Pierce GST Agarose.
This GST resin consists of purified glutathione S-transferase protein from Schistosoma japonicum that has been immobilized on crosslinked 6% beaded agarose. Antibody molecules in that cross-react with the GST portion of a recombinant fusion protein can be removed from the desired protein-specific antibody by passing antiserum over a GST agarose column. GST antibodies will bind to the immobilized GST, whereas antibodies against other fusion proteins will pass through the column unrestrained.Highlights:
Specific – provides purification of target antibodies from those directed against the GST tag
Reusable – bound antibodies can be eluted with 6M guanidinium chloride allowing for 10 purifications with each column or aliquot of resin
Two formats – choose either pre-packed columns or resin slurry for use at a variety of scales
Specifications
Agarose | |
Agarose Resin, Affinity | |
GST Protein | |
Pierce GST Agarose | |
5 mL |
Pierce™ | |
Bottle | |
Anti-GST Antibody | |
4°C |
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For Research Use Only. Not for use in diagnostic procedures.