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Hydrophobic Interaction Chromatography Reagents and Supplies

Hydrophobic Interaction Chromatography Reagents and Supplies

Chemical reagents and products designed for hydrophobic interaction chromatography (HIC); HIC is a separation technique that takes advantage of hydrophobicity to separate proteins in a sample/solution.
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1

Cytiva HiTrap™ Capto™ HIC Selection Kit

Five Capto™ Hydrophobic Interaction Chromatography Resins in HiTrap™ Columns for resin selection and screening protein purification conditions

2

Cytiva HiTrap™ Butyl HP Columns

Prepacked columns for convenient and fast separations. Cytiva HiTrap™ Butyl HP Columns are used for small-scale protein purification as well as for screening of binding and elution conditions.

Particle Size 24 to 44 µm
Ligand Type Butyl
Height (Metric) 30 mm
pH Range 3 to 13
Product Type HiTrap Butyl HP
Material Polypropylene
Storage Requirements 4°C to 30°C, 20% Ethanol
For Use With (Application) For Screening of Selectivity, Binding and Elution Conditions, as Well as Small Scale Purifications
Support Type Cross-linked Agarose
Media Type Butyl Sepharose
Max. Pressure 5 bar [0.5 MPa] (70 psi)
Height (English) 1.181 in.
3

Cytiva HiTrap Capto Butyl ImpRes

A ready-to-use column prepacked with BioProcess Capto Butyl ImpRes resins for screening and small-scale protein purification using hydrophobic interaction chromatography.

4

Cytiva HiTrap™ HIC Sepharose™ Selection Kit

Use for laboratory and intermediate process-scale separations and final-step purifications.  Cytiva Life Sciences™ HiTrap™ HIC Sepharose™ Selection Kit takes full advantage of hydrophobic interaction chromatography providing highest resolution. 

5

Cytiva Phenyl Sepharose™ CL-4B Gel

For immunoglobulin purification and fractionation as well as immunoprecipitation procedures

6

Cytiva HIC (Hydrophobic Interaction Chromatography) Media

Features high chemical stability, properties to increase productivity and epoxy coupling

Particle Size 75 µm
Flow Rate 600 cm/Hoµr
Binding Capacity 27 mg BSA/mL
Storage Buffer 20% Ethanol
pH Range 3 to 13
Quantity 25 mL
Storage Requirements 4°C to 30°C
For Use With (Application) For Used in The Capture and Intermediate Stages of Protein PurifiCatIon
Height (Metric) Bed 20 cm
Media Type Highly Cross-Linked Agarose
Max. Pressure 300 kPa
7

Cytiva Untagged Column Package

Combines cation and anion exchange with size exclusion chromatography for purification of most untagged or native proteins

8

Cytiva HiTrap™ Phenyl FF (low sub) Columns

Prepacked columns for convenient and fast separations

Particle Size 45 to 165 µm
Ligand Type Phenyl
Height (Metric) 25 mm
pH Range 3 to 13
Product Type HiTrap Phenyl FF (Low Sub)
Material Polypropylene
Storage Requirements 4°C to 30°C, 20% Ethanol
For Use With (Application) For Screening of Selectivity, Binding and Elution Conditions, as Well as Small Scale Purifications
Support Type 6% Cross-linked Agarose
Media Type Phenyl Sepharose 6 Fast Flow (low sub)
Max. Pressure 5 bar [0.5 MPa] (70 psi)
Height (English) 0.984 in.
9

Cytiva HiTrap™ Octyl FF Columns

Prepacked columns for convenient and fast separations

Particle Size 45 to 165 µm
Ligand Type Octyl
Height (Metric) 25 mm
pH Range 3 to 13
Product Type HiTrap Octyl FF
Material Polypropylene
Storage Requirements 4°C to 30°C, 20% Ethanol
For Use With (Application) For Screening of Selectivity, Binding and Elution Conditions, as Well as Small Scale Purifications
Support Type 4% Cross-linked Agarose
Media Type Octyl Sepharose 4 Fast Flow
Max. Pressure 5 bar [0.5 MPa] (70 psi)
Height (English) 0.984 in.
10

Cytiva HiTrap™ Butyl-S FF Columns

Designed for purification of relatively strong hydrophilic molecules at low salt concentrations

Particle Size 45 to 165 µm
Ligand Type Butyl-S
pH Range 3 to 13
Product Type HiTrap Butyl-S FF column
Material Polypropylene
Support Type 6% Cross-linked Agarose
11

Cytiva HiTrap™ Phenyl FF (high sub) Columns

Prepacked columns for convenient and fast separations

Particle Size 45 to 165 µm
Ligand Type Phenyl
Height (Metric) 25 mm
pH Range 3 to 13
Product Type HiTrap Phenyl FF (High Sub)
Material Polypropylene
Storage Requirements 4°C to 30°C, 20% Ethanol
For Use With (Application) For Screening of Selectivity, Binding and Elution Conditions, as Well as Small Scale Purifications
Support Type 6% Cross-linked Agarose
Media Type Phenyl Sepharose 6 Fast Flow (high sub)
Max. Pressure 5 bar [0.5 MPa] (70 psi)
Height (English) 0.984 in.
12

Cytiva HiTrap Capto Phenyl ImpRes

HiTrap Capto Phenyl ImpRes is prepacked with BioProcess Capto Phenyl ImpRes resins for screening and small-scale protein purifications using hydrophobic interaction chromatography.

13

Cytiva HiTrap™ Butyl FF Columns

Prepacked columns for convenient and fast separations

Particle Size 45 to 165 µm
Ligand Type Butyl
Height (Metric) 25 mm
pH Range 3 to 13
Product Type HiTrap Butyl FF
Material Polypropylene
Storage Requirements 4°C to 30°C, 20% Ethanol
For Use With (Application) For Screening of Selectivity, Binding and Elution Conditions, as Well as Small Scale Purifications
Support Type 4% Cross-linked Agarose
Media Type Butyl Sepharose 4 Fast Flow
Max. Pressure 5 bar [0.5 MPa] (70 psi)
Height (English) 0.984 in.
14

Cytiva RESOURCE™ Columns for HIC

Excellent for polishing in a purification process

Particle Size 15 to 15 µm
Includes Connectors for high- and low-pressure chromatography equipment and instructions
Height (Metric) 10 cm
Flow Rate 150 to 900 cm/hr
Diameter (English) Inner 3.937 in.
pH Range 2 to 12
Diameter (Metric) Inner 10 cm
Quantity 1 mL
Material PEEK
Storage Requirements 4°C to 30°C, 20% Ethanol
Support Type Polystyrene/Divinylbenzene
Max. Pressure 15 bar
Height (English) 3.937 in.
15

Cytiva HiTrap™ IgG Sepharose FF

Cytiva HiTrap™ IgG Sepharose FF is easy to use with a syringe, peristaltic pump, or a chromatography system.