Buffers and Standards

Hydrogen ion buffers comprising a mixture of a weak acid and its conjugate base or vice-versa and used to stabilize pH; also diluents, washing solutions, and standard-value solutions used for a broad array of scientific calibration purposes.

The buffers used to calibrate pH meters may be certified and/or traceable to the National Institute of Standards and Technology (NIST). These buffers may also be color-coded for easy identification:

Red: pH 4.0
Yellow: pH 7.0
Blue: pH 10.0

Chromatography Standards (10,324)

General Chemistry Solutions (4,481)

Spectroscopy Standards (3,080)

Matrix Blanks and Standardization Solutions (2,729)

Life Science Buffers (1,895)

Electrochemistry Buffers (1,240)

Environmental Testing Standards (1,236)

Electrochemistry Standards (711)

Karl Fischer Buffers and Reagents (346)

Quality Control and Proficiency Samples (156)

Viscosity Standards (130)

Kjeldahl Buffers and Reagents (63)

Matrix Modifiers (27)

Isotope Standards (21)

Osmolality Standards (11)

Polarimetric Standards (3)

Gel Filtration Standards (2)

Smoke Point Reference (2)

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Buffer Solution, pH 4.00, Color-Coded Red (Certified), Fisher Chemical™

pH/temperature conversions given on label

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Specifications

Boiling Point	100°C
Description	pH/temperature conversions given on label.
Color	Red
Traceability to NIST	Traceable to NIST.
Physical Form	Liquid
Chemical Name or Material	Buffer Solution, pH 4.00, Color-Coded Red
Grade	Certified
Identification	Pass Test
ChemAlert Storage Symbol	Gray
Density	1.0g/cm³
Absorbance	Pass Test
Concentration or Composition (by Analyte or Components)	Potassium Hydrogen Phthalate
CAS	16423-68-0
Health Hazard 3	Emergency Overview Contains a known or suspected carcinogen. The toxicological properties have not been fully investigated. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Do not induce vomiting. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
MDL Number	MFCD00146206
Health Hazard 2	CAUTION!
Solubility Information	Soluble in water
pH	4.0
Specific Gravity	1
Melting Point	0°C

Buffer Solution, pH 4.00 (Certified), Fisher Chemical™

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CAS	67-56-1
Health Hazard 3	Emergency Overview Contains a known or suspected carcinogen. The toxicological properties have not been fully investigated. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Do not induce vomiting. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
MDL Number	MFCD00146206
Health Hazard 2	CAUTION!
Color	Undesignated
Traceability to NIST	Traceable to NIST.
Physical Form	Liquid
pH	4.0
Chemical Name or Material	Buffer Solution, pH 4.00
Grade	Certified
Identification	Pass Test
ChemAlert Storage Symbol	Gray
Density	1.0054g/cm³

Buffer Solution, pH 10.00 (Certified), Fisher Chemical™

Potassium Carbonate/Potassium Tetraborate/Potassium Hydroxide/Disodium EDTA Dihydrate

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Specifications

CAS	12228-88-5
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Handle in accordance with good industrial hygiene and safety practice. Wear personal protective equipment. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:1 Flammability:0 Instability:0
MDL Number	MFCD00146206
Health Hazard 2	CAUTION!
Color	Colorless
Traceability to NIST	Traceable to NIST.
Physical Form	Liquid
pH	10
Chemical Name or Material	Buffer Solution, pH 10.00
Grade	Certified
Identification	Pass Test
ChemAlert Storage Symbol	Gray

Buffer Solution, pH 7.00, Color-Coded Yellow (Certified), Fisher Chemical™

Pricing & Availability
Specifications

Boiling Point	100°C
Description	Potassium Phosphate Monobasic/Sodium Hydroxide. pH/temperature conversions given on label.
Color	Yellow
Traceability to NIST	Traceable to NIST.
Physical Form	Liquid
Chemical Name or Material	Buffer Solution, pH 7.00, Color-Coded Yellow
Grade	Certified
Identification	Pass Test
ChemAlert Storage Symbol	Gray
Density	1.0g/cm³
Vapor Pressure	760mmHg at 20°C
Absorbance	Pass Test
CAS	1934-21-0
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Handle in accordance with good industrial hygiene and safety practice. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Obtain medical attention. . . NFPA Health:1 Flammability:0 Instability:0
MDL Number	MFCD00134130
Health Hazard 2	CAUTION!
Solubility Information	Soluble in water
pH	7.00
Specific Gravity	1
Melting Point	0°C

Buffer Solution, pH 7.00 (Certified), Fisher Chemical™

Potassium Phosphate Monobasic/Sodium Hydroxide

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Specifications

Color	Colorless
Traceability to NIST	Traceable to NIST.
Physical Form	Liquid
Chemical Name or Material	Buffer Solution, pH 7.00
Grade	Certified
Identification	Pass Test
ChemAlert Storage Symbol	Gray
Density	1.003g/cm³
Vapor Pressure	14mmHg at 20°C
CAS	7732-18-5
MDL Number	MFCD00134130
pH	7.00
Synonym	Chlorine Titrimeter Reagent
Melting Point	0°C

Buffer Solution, pH 10.00, Color-Coded Blue (Certified), Fisher Chemical™

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Specifications

Boiling Point	100°C
Color	Blue
Traceability to NIST	Traceable to NIST.
Physical Form	Liquid
Chemical Name or Material	Buffer Solution, pH 10.00, Color-Coded Blue
Grade	Certified
Identification	Pass Test
ChemAlert Storage Symbol	Gray
Absorbance	Pass Test
CAS	1310-58-3
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:1 Flammability:0 Instability:0
MDL Number	MFCD00146206
Health Hazard 2	CAUTION!
pH	10.0
Specific Gravity	1.013 at 25°C
Melting Point	0°C

Buffer Solution, pH 5.00 (Certified), Fisher Chemical™

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Specifications

Boiling Point	100°C
CAS	7732-18-5
MDL Number	MFCD00146206
Traceability to NIST	Traceable to NIST.
Packaging	Poly Bottle
Physical Form	Liquid
pH	5.0
Chemical Name or Material	Buffer Solution, pH 5.00
Grade	Certified
Identification	Pass Test
ChemAlert Storage Symbol	Gray
Melting Point	0°C

TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™

Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT

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Specifications

Name Note	50X Solution
Concentration or Composition (by Analyte or Components)	Tris (24%), Acetic Acid (5.0%), and EDTA (<2%).
CAS	6850-28-8
Protease	Protease free
Physical Form	Liquid
Grade	Electrophoresis
Synonym	TAE
Purity Grade Notes	DNase-, RNase- and Protease-Free
DNase	DNase free
Recommended Storage	RT
Filtered Through	Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol	Gray

Buffer Solution, pH 8.00 (Certified), Fisher Chemical™

Potassium Phosphate Monobasic/Sodium Hydroxide

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Specifications

CAS	7732-18-5
Health Hazard 3	Emergency Overview Irritating to eyes and skin. The toxicological properties have not been fully investigated. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Do not induce vomiting. Obtain medical attention. NFPA Health:2 Flammability:0 Instability:0
MDL Number	MFCD00147253
Health Hazard 2	WARNING!
Traceability to NIST	Traceable to NIST.
Physical Form	Liquid
pH	8.0
Chemical Name or Material	Buffer Solution, pH 8.00
Grade	Certified
Identification	Pass Test
ChemAlert Storage Symbol	Gray

PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™

Commonly used buffer

Pricing & Availability
Specifications

Content And Storage	Room temperature
Form	Liquid

Buffer Solution, pH 9.00 (Certified), Fisher Chemical™

Boric Acid/Potassium Chloride/Sodium Hydroxide

Pricing & Availability
Specifications

Boiling Point	100°C
CAS	7732-18-5
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:1 Flammability:0 Instability:0
MDL Number	MFCD00146206
Health Hazard 2	CAUTION!
Traceability to NIST	Traceable to NIST.
Physical Form	Liquid
pH	9.00
Chemical Name or Material	Buffer Solution, pH 9.00
Grade	Certified
Identification	Pass Test
ChemAlert Storage Symbol	Gray
Melting Point	0°C

Iodine Solution, Wijs, Fisher Chemical™

CAS: 7553-56-2 Molecular Formula: I2 Molecular Weight (g/mol): 253.81 MDL Number: MFCD00011355 MFCD00164163 InChI Key: PNDPGZBMCMUPRI-UHFFFAOYSA-N Synonym: iodine,diiodine,iodine crystals,iodine sublimed,tincture iodine,vistarin,eranol,iodio,iodine solution,iode PubChem CID: 807 ChEBI: CHEBI:17606 IUPAC Name: diiodine SMILES: II

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Specifications

PubChem CID	807
CAS	7553-56-2
Molecular Weight (g/mol)	253.81
ChEBI	CHEBI:17606
MDL Number	MFCD00011355 MFCD00164163
SMILES	II
Synonym	iodine,diiodine,iodine crystals,iodine sublimed,tincture iodine,vistarin,eranol,iodio,iodine solution,iode
IUPAC Name	diiodine
InChI Key	PNDPGZBMCMUPRI-UHFFFAOYSA-N
Molecular Formula	I2

Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
SMILES	C(C(CO)(CO)N)O
Synonym	Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

Buffer Solution, pH 6.00 (Certified), Fisher Chemical™

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Specifications

Boiling Point	100°C
Color	Undesignated
Traceability to NIST	Traceable to NIST.
Physical Form	Liquid
Chemical Name or Material	Buffer Solution, pH 6.00
Grade	Certified
Identification	Pass Test
ChemAlert Storage Symbol	Gray
CAS	1310-73-2
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Handle in accordance with good industrial hygiene and safety practice. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Obtain medical attention. . . NFPA Health:1 Flammability:0 Instability:0
MDL Number	MFCD00134130
Health Hazard 2	CAUTION!
pH	6.00
Melting Point	0°C

TAE Buffer, Tris-Acetate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents

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Specifications

Name Note	10X Solution
Concentration or Composition (by Analyte or Components)	Tris (<5.0%), Acetic Acid (1.0%), and EDTA (<1%).
CAS	77-86-1
Protease	Protease free
Physical Form	Liquid
Chemical Name or Material	Tris-Acetate-EDTA
Grade	Electrophoresis
Synonym	TAE
Purity Grade Notes	DNase-, RNase- and Protease-Free
DNase	DNase free
Recommended Storage	RT
Filtered Through	Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol	Gray

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Buffers and Standards

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